Figure 2.
Characterization of the Loss-of-Function knat6 and knat2 Mutant Alleles.
(A) RT-PCR analysis using KNAT6 gene-specific primers. After 38 cycles, the PCR products were transferred to a nylon membrane and hybridized with a 33P-labeled KNAT6 probe. Amplification occurred in wild-type seedlings (lane 1) but not in knat6-1 and knat6-2 alleles (lanes 2 and 3, respectively), indicating that both alleles are null. Control amplification of APT1 indicates that equal amounts of cDNA were present in each sample.
(B) RT-PCR analysis using KNAT2 gene-specific primers. After 40 cycles, the PCR products were transferred to a nylon membrane and hybridized with a 33P-labeled KNAT2 probe. Amplification occurred in wild-type seedlings (lane 1) but not in the knat2-5 allele (lane 2), indicating that the knat2-5 allele is null. Control amplification of APT1 indicates that equal amounts of cDNA were present in each sample.