Table I.
Blocking of CD70 expression on TIL-inhibited proliferation of CD8+ cells
| Anti-IgGa | Anti-CD70 (%) | Inhibitionb | pc | |
|---|---|---|---|---|
| CD70 expressiond | 41.8 ± 1.8 | 0.2 ± 0.0 | 98.1 ± 0.0 | <0.001 |
| Proliferatione | 36.0 ± 2.8 | 21.3 ± 0.7 | 37.9 ± 1.5 | <0.005 |
a
Patient TIL consisting of ~98% CD8+ T cells were cultured at a concentration of 1 × 106 cells/ml with 3,000 IU/ml IL-2 for 2 days with either an anti-CD70 or an isotype-matched control Ab at a concentration of 6.8 μg/ml.
b
Percentage of Inhibition = value(anti-IgG) minus; value(anti-CD70)/value(anti-IgG) × 100%.
c
The significance of differences was evaluated using a two-tailed Mann-Whitney test.
d
The expression of CD70 was analyzed by FACS analysis of gated CD8+ T cells.
e
Cell cycle analysis was carried out to determine the extent of proliferation of T cells cultures. Values indicate the percentage of CD8+ T cells in S phase.