FIG. 2.
Arabidopsis PBRP is ubiquitously expressed. (A) Northern blot analysis of total RNA (∼20 μg) prepared from A. thaliana roots (lanes R), leaves (lanes L), stems (lanes S), flower buds (lanes FB), flowers (lanes F), and siliques (lanes Si) with PBRP cDNA as a probe. Ethidium bromide staining of the corresponding denaturing gel is shown as loading control. (B) Arabidopsis PBRP is ubiquitously expressed as revealed by GUS reporter expression. Transgenic seedlings expressing the GUS reporter gene under the control of A. thaliana PBRP (AtPBRP) promoter were grown for 1 week under light or dark conditions and then stained for GUS activity. Flowers and siliques from mature plants were also analyzed by histochemical assay.