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Presence of clathrin and AP-1 on GGA1-containing carriers. MDCK cells were simultaneously transfected with constructs encoding YFP-clathrin and CFP-GGA1 (A) or GFP-γ1 (GFP-AP-1) and CFP-GGA1 (B). Confocal microscopy images were acquired every 3 s. Arrows indicate vesicular carriers budding from the TGN, whereas arrowheads point to immobile structures. The yellow color in the merged images indicates colocalization between clathrin and GGA1 (A) or GGA1 and AP-1 (B). Bars, 2 μm.
