Figure 3.
(A) Expression patterns of selected genes for TCA cycle and glyoxylate bypass enzymes. Eight representative genes are shown in this figure, and table A lists the results of the remaining genes. Expression patterns for the following genes are shown: gltA, citrate synthase II; acnA, aconitase; icd, isocitrate dehydrogenase; sucC, succinyl-CoA synthetase, β-chain; sucD, succinyl CoA synthetase, α-chain; mdh, malate dehydrogenase; aceA, isocitrate lyase; and aceB, malate synthase. Genes specific to the TCA cycle are green, glyoxylate bypass genes are red, and genes shared by both TCA cycle and glyoxylate bypass are blue. Genes not represented here include: three subunits of 2-oxoglutarate dehydrogenase complex [lpd, lipoamide dehydrogenase (DR2526); sucA, 2-oxoglutarate dehydrogenase (DR0287); and sucB, dihydrolipoamide succinyltransferase (DR0083)] are substantially down-regulated like icd (DR1540). Malate synthase (DRA0277) of the glyoxylate bypass is slightly down-regulated. Four subunits of fumarate dehydrogenase complex [sdhB, fumarate/succinate dehydrogenase type iron sulfur protein (DR0951); sdhA, succinate dehydrogenase Rossman fold oxidoreductase (DR0952); shhC and sdhD, small succinate dehydrogenase associated proteins (DR0953 and DR0954); and fumC, fumarase (DR2627)] do not show significant changes in their expression levels during postirradiation recovery. (B) Expression patterns of the genes for nitrogen metabolism. Gene abbreviations: glnB, P-II nitrogen metabolism regulatory protein; amtB, ammonium transporter protein; gltD and gltB, glutamate synthase subunits. (C) Expression patterns of the genes for carbohydrate utilization. Gene abbreviations: tktA, transketolase; fruK, 1-phosphofructokinase.