TABLE 3.
HIS3-GUS expression phenotypes of cofactor mutants
| Relevant genotype | Cofactor complex | β-Glucuronidase activity (% of WT) fora:
|
|
|---|---|---|---|
| Uninduced HIS3-GUS | Induced HIS3-GUS | ||
| gcn4Δ | Activator | 55 | 11 |
| mbf1Δ | NAb | 76 | 27∗ |
| ada2Δ | ADA, SAGA | 86 | 16∗ |
| ada3Δ | ADA, SAGA | 143 | 20∗ |
| gcn5Δ | ADA, SAGA | 29 | 6.4∗ |
| spt8Δ | SAGA | 289 | 84 |
| spt3Δ | SAGA | 141 | 29∗ |
| ada1Δ | SAGA | 149 | 20∗ |
| ada5Δ | SAGA | 235 | 32∗ |
| spt7Δ | SAGA | 46 | 9.1∗ |
| tfg3Δ | Multiplec | 113 | 58 |
| rsc1Δ | RSC | 15 | 4.2∗ |
| rsc2Δ | RSC | 23 | 49 |
| swi2Δ | SWI/SNF | 523 | 169 |
| swi3Δ | SWI/SNF | 43 | 25 |
| snf5Δ | SWI/SNF | 363 | 117 |
| snf6Δ | SWI/SNF | 147 | 82 |
| snf11Δ | SWI/SNF | 112 | 83 |
| swp73Δ | SWI/SNF | 437 | 129 |
| cdc73Δ | Paf1 complex | 117 | 42∗ |
| hpr1Δ | Paf1 complex, THO/TREX | 13 | 3.3∗ |
| ccr4Δ | CCR4-NOT, Paf1 complex | 25 | 10∗ |
| not3Δ | CCR4-NOT | 58 | 52 |
| caf16Δ | CCR4-NOT | 76 | 40 |
| not4Δ | CCR4-NOT | 67 | 51 |
| caf40Δ | CCR4-NOT | 58 | 33 |
| caf130Δ | CCR4-NOT | 56 | 46 |
| caf4Δ | CCR4-NOT | 86 | 54 |
| caf17Δ | CCR4-NOT | 97 | 64 |
| dbf2Δ | CCR4-NOT | 40 | 20∗ |
| dhh1Δ | CCR4-NOT | 16 | 12 |
| not5Δ | CCR4-NOT | 67 | 39 |
| caf1Δ | CCR4-NOT | 53 | 10∗ |
| srb11Δ | SRB/MED, CCR4-NOT | 140 | 77 |
| srb9Δ | SRB/MED, CCR4-NOT | 53 | 12∗ |
| srb10Δ | SRB/MED, CCR4-NOT | 227 | 73 |
| pgd1Δ | SRB/MED | 469 | 259 |
| med2Δ | SRB/MED | 97 | 43∗ |
| rox3Δ | SRB/MED | 92 | 21∗ |
| sin4Δ | SRB/MED, Paf1 complex | 253 | 119 |
| ga111Δ | SRB/MED, Paf1 complex | 145 | 55∗ |
At least three independent transformants of each strain harboring the reporter plasmid pKN7 were grown under inducing and noninducing conditions (presence and absence of SM) as described in Materials and Methods, and β-glucuronidase activity was assayed in the cell extracts. Mean values were calculated from results that yielded standard deviations of 20% or less and expressed as percentages of the corresponding values measured in the WT parental strains. Results in italics are 200% of the WT value or greater. Underlined results are <68% of the WT value under inducing conditions and show reductions relative to the WT that are 2-fold (boldface with an asterisk), 1.5-fold (boldface only), or less than 1.5-fold greater under inducing than noninducing conditions.
NA, not applicable.
Tfg3p is present in the SWI/SNF, TFIID, NuA3, and TFIIF complexes.