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. 2003 Apr;23(8):2800–2820. doi: 10.1128/MCB.23.8.2800-2820.2003

TABLE 3.

HIS3-GUS expression phenotypes of cofactor mutants

Relevant genotype Cofactor complex β-Glucuronidase activity (% of WT) fora:
Uninduced HIS3-GUS Induced HIS3-GUS
gcn4Δ Activator 55 11
mbf1Δ NAb 76 27∗
ada2Δ ADA, SAGA 86 16∗
ada3Δ ADA, SAGA 143 20∗
gcn5Δ ADA, SAGA 29 6.4∗
spt8Δ SAGA 289 84
spt3Δ SAGA 141 29∗
ada1Δ SAGA 149 20∗
ada5Δ SAGA 235 32∗
spt7Δ SAGA 46 9.1∗
tfg3Δ Multiplec 113 58
rsc1Δ RSC 15 4.2∗
rsc2Δ RSC 23 49
swi2Δ SWI/SNF 523 169
swi3Δ SWI/SNF 43 25
snf5Δ SWI/SNF 363 117
snf6Δ SWI/SNF 147 82
snf11Δ SWI/SNF 112 83
swp73Δ SWI/SNF 437 129
cdc73Δ Paf1 complex 117 42∗
hpr1Δ Paf1 complex, THO/TREX 13 3.3∗
ccr4Δ CCR4-NOT, Paf1 complex 25 10∗
not3Δ CCR4-NOT 58 52
caf16Δ CCR4-NOT 76 40
not4Δ CCR4-NOT 67 51
caf40Δ CCR4-NOT 58 33
caf130Δ CCR4-NOT 56 46
caf4Δ CCR4-NOT 86 54
caf17Δ CCR4-NOT 97 64
dbf2Δ CCR4-NOT 40 20∗
dhh1Δ CCR4-NOT 16 12
not5Δ CCR4-NOT 67 39
caf1Δ CCR4-NOT 53 10∗
srb11Δ SRB/MED, CCR4-NOT 140 77
srb9Δ SRB/MED, CCR4-NOT 53 12∗
srb10Δ SRB/MED, CCR4-NOT 227 73
pgd1Δ SRB/MED 469 259
med2Δ SRB/MED 97 43∗
rox3Δ SRB/MED 92 21∗
sin4Δ SRB/MED, Paf1 complex 253 119
ga111Δ SRB/MED, Paf1 complex 145 55∗
a

At least three independent transformants of each strain harboring the reporter plasmid pKN7 were grown under inducing and noninducing conditions (presence and absence of SM) as described in Materials and Methods, and β-glucuronidase activity was assayed in the cell extracts. Mean values were calculated from results that yielded standard deviations of 20% or less and expressed as percentages of the corresponding values measured in the WT parental strains. Results in italics are 200% of the WT value or greater. Underlined results are <68% of the WT value under inducing conditions and show reductions relative to the WT that are 2-fold (boldface with an asterisk), 1.5-fold (boldface only), or less than 1.5-fold greater under inducing than noninducing conditions.

b

NA, not applicable.

c

Tfg3p is present in the SWI/SNF, TFIID, NuA3, and TFIIF complexes.