Fig. 6.
Impaired maturation of CP neurons in GLAST−/−/GLT1−/− mutants. (A) At E16, pyramidal-like retrogradely labeled cells are observed in the CP of WT mice after DiI injection in the thalamus. (B) In mutants, the morphology and neurite extension of the retrogradely labeled cells were affected. (C and D) Coronal sections of neocortex at E14 from WT mice (C) and GLAST−/−/GLT1−/− mutants (D) stained with hematoxylin. (E–H) SEM analysis was performed with E14 cortices. Although the hematoxylin staining could not reveal impaired maturation of CP neurons in mutants at E14, SEM analysis revealed that the cellular morphology was affected in GLAST−/−/GLT1−/− mutants (F) compared with WT (E). In contrast, the cellular morphology in the VZ was comparable in WT (G) and mutant (H) E14 cortices. DM, double mutants. (Scale bars: A and C, 100 μm; E and G, 5 μm.)