FIG. 1.
Analysis of cDNA inserts from phagemid clones and 5′ RACE. (A) Phagemids obtained after screening of the HBMEC cDNA library with Ecgp-Ab were digested with EcoRI and separated on a 1% agarose gel. A 1-kb DNA marker is loaded on the left. (B) The DNA contents from the agarose gel were transferred to a nylon membrane and subjected to Southern hybridization using a degenerate oligonucleotide probe obtained from the N-terminal amino acid sequence of gp65. (C) Schematic representation of the overlapping regions of different cDNA clone sequences. (D) Agarose gel electrophoresis of the 5′ RACE product using RNAs of HBMEC and HUVEC. The primers and DNA supplied by the manufacturer were used as a positive control (+ control), and HBMEC RNA without primers was used as a negative control (− control).