TABLE 1.
E. coli strains and plasmids
| Strain or plasmid | Relevant properties | Source or reference |
|---|---|---|
| Strains | ||
| E2348/69 wt | O127:H6 clinical isolate | 39 |
| CVD206 | eae mutant of E2348/69 | 18 |
| CVD452 | escN mutant of E2348/69 | 33 |
| AGT01 | fliC mutant of E2348/69 | 29 |
| AGT02 | fliC (pfliC); AGT01 complemented with cloned fliC from E2348/69 | 29 |
| AGT03 | motB mutant of E2348/69 | 29 |
| E10 wt | O119:H6 clinical isolate | 29 |
| AGT04 | fliC mutant of E10 | 29 |
| B171 | O111:NM; clinical isolate; nonflagellated | 29 |
| EAEC O42 wt | Clinical isolate | 50 |
| EAEC O42 fliC | fliC mutant of EAEC O42 | 50 |
| ORN172 | K-12, with type I pilus genes deleted | 29 |
| E28 | O86:H34; clinic isolate | 29 |
| EHEC 86-24 O157:H7 | H7 | 29 |
| DH5α | K-12 cloning host strain | Stratagene |
| Plasmids | ||
| pQE30 | His tag expression vector | Qiagen |
| pXZ13 | pQE30 containing fliC from E2348/69 | This study |
| pREP4 | Repressor plasmid containing lacI | Qiagen |