Table 1.
Purification of serine racemase
| Fraction | Protein, mg | Specific activity, μmol l-Ser/mg per h | Fold purification | Total activity | Yield, % |
|---|---|---|---|---|---|
| Homogenate | 4,744 | ND | — | — | — |
| (NH4)2SO4 fractionation | 624 | 0.0004 | 1 | 0.249 | 100 |
| Butyl-sepharose | 34 | 0.003 | 7.5 | 0.102 | 41 |
| Q-sepharose | 6.6 | 0.029 | 73 | 0.191 | 77 |
| Mono Q | 0.22 | 0.833 | 2,082 | 0.183 | 73 |
| Hydroxyapatite | 0.015 | 5.0 | 12,500 | 0.075 | 30 |
Enzyme was purified and fractions were assayed as described. Data represent a typical purification, which was repeated six times with similar results. ND, not detected.