Figure 4.
Dissociation of Med6 and core polymerase from the mediator complex. Shown is an immunoblot of Bio-Sil 400 SEC fractions from wild-type (W303–1A) and med1 (H707) yeast cells. One fraction from each UNO Q-1 column containing both the mediator subunits and the core polymerase was analyzed by gel filtration on a Bio-Sil 400 SEC column. The fractions from the gradient were separated on a 10% SDS/PAGE gel and were immunoblotted with antibodies directed against individual proteins as indicated to the left.