Figure 3.
PYR complex contains at least four known mammalian SWI/SNF subunits. Gel supershift assay using MEL nuclear extract and labeled δ60ym DNA as probe. Nuclear extract was preincubated for 1 hr at room temperature with antibodies to known SWI/SNF complex subunits (lanes 2–7) and other nuclear factors, two of which are shown (lanes 8 and 9). Strong supershifts are seen with anti-BAF57, anti-INI1, and anti-BAF60a antibodies, with a weaker supershift using antibody to BAF170. No clear supershift is seen with anti-BRG1, anti-BAF155, anti-HMG I(Y), or anti-RbAp46.