FIG. 2.
Plasma membrane and cilia localization of PC2 in IMCD and MDCK cells. Double labeling of IMCD (A to C) or MDCK (J to L) cells by the anti-PC2 antibody 96525 (A, J) and anti-p120, a cell plasma membrane-associated protein (B), or anti-E-cadherin, a plasma membrane protein (K), localizes PC2 to cell plasma membrane (C, L). Double labeling of IMCD (D to F) or MDCK (M to O) cells by 96525 (D, M) and a cilia marker, acetylated α-tubulin (E, N), showed that PC2 is also localized to the monocilia in these cells. Preincubation with a PC2-specific peptide completely blocked the PC2 signal (G, P) but not the membrane or cilia marker (H, I, Q, R).