Figure 7.
Orientation of ACAT1-465 and ACAT2-443 in the ER membrane. (A) ACAT1-465 and ACAT2-443 mRNA were translated in vitro in the presence and absence of rough microsomes and glycosylation inhibitor as described in Figure 2B. (B) AC29 cells were transiently transfected with ACAT1-465 (a and b) or ACAT2-443 (c and d). After permeabilization with digitonin (a and c) or saponin (b and d), cells were stained with anti-FLAG M2 C-term antibody as described in Figure 2A. (C) ACAT1-465 and ACAT2-443 membrane topology models.