Fig. 2.
yaa contains a translocation of the X-linked Tlr7 gene. (A) Expression-level fold changes of the indicated X chromosome genes in B cells from 4- to 6-month-old mice of the different yaa strains relative to B6 controls, as determined by using Illumina BeadChip arrays (Upper). (Lower) Positions of the changed genes (bold) on the F5 band of mouse chromosome X according to www.ensembl.org. (B) Quantitative real-time PCR analyses of Tlr7 expression in purified B cells from different strains, confirming up-regulation in Tlr7 expression in yaa-bearing mice relative to B6. Error bars represent SEM. (C) Two-color FISH analyses were performed by using FITC-labeled RP23-92P6 BAC DNA (green) and Spectrum Orange-labeled RP24-208N6 BAC DNA (pink). Hybridization was done to metaphase chromosome spreads of splenic lymphocyte cultures from 7-week-old B6 and B6.yaa mice. Hybridization of respective probes is indicated: arrows, RP23-92P6 (green); arrowheads, RP24-208N6 (pink).