FIG. 2.
Cystine-H2O2 treatment generates abundant DNA damage. Log-phase E. coli AB1157 cells were treated with either 0.5 mM cystine, 2.5 mM H2O2, or cystine and H2O2, or left untreated as a control (con). Total genomic DNA was isolated, and qPCR was performed, using equivalent amounts of template DNA for each reaction mixture. PCR products were scanned, and the relative fluorescence was normalized to the untreated control. Values are the means and standard deviations (error bars) from three experiments.