Table 2.
Plasmids used in this study
| Plasmid | Characteristics | Reference or source |
|---|---|---|
| pAGX1 | PACT1-EGFP-TTDH3 URA3 CEN6 | Ozaki-Kuroda et al. (2001) |
| pAGX1-BNR1 | PACT1-EGFP-BNR1-TTDH3 URA3 CEN6 | A gift from K. Ozaki-Kuroda |
| pKU65 | vps27Δ::HIS3 | A gift from K. Umebayashi |
| p406S2G | SPA2-GFP URA3 | Arkowitz and Lowe (1997) |
| pSRG92 | myc-VPS21 URA3 2μm | Gerrard et al. (2000) |
| pSL2099 | PGAL1-STE3::myc LEU2 CEN6 | Davis et al. (1993) |
| SEC3-GFP 3-1 | SEC3-GFP URA3 CEN6 | Finger et al. (1998) |
| pDAb204 | PACT1-GFP-BUD6 URA3 CEN4 | Amberg et al. (1997) |
| YEplac181 | LEU2 2μm | Gietz and Sugino (1988) |
| pRS314-CDC3G | CDC3-GFP TRP1 CEN | A gift from M. Iwase |
| pKT1029 | MYO5 LEU2 2μm | This study |
| pKT1257 | LAS17 LEU2 2μm | This study |
| pKT1241 | BNI1-EGFP-KanMX6 | This study |
| pKT1258 | UBP5 LEU2 2μm | This study |
| pKT1259 | CDC50 LEU2 2μm | This study |
| pKT1265 | CDC50-EGFP URA3 CEN | This study |
| pKT1266 | CDC50-EGFP LEU2 2μm | This study |