Figure 5.
Ykt6-containing membranes do not overlap significantly with markers of the secretory pathway. PC12 cells were fixed and stained with anti-ykt6 antibodies and FITC-labeled secondary antibodies (A, D, G, J, and M) and costained with the following primary antibodies followed by Texas Red–labeled secondary antibodies: GM130 (B), rbet1 (E), VAMP 2 (H) and synaptotagmin I (K and N). Green and red images from the same field were merged (C, F, I, and L). (M and N) Single deconvolved optical sections. Insets show DIC images of the respective fields. Detail in I shows a neurite terminal containing distinct, ykt6- and VAMP2-containing structures. Arrows in J–N point to the few colocalizing spots. Bars, 10 μm.