Figure 3.
Rat ykt6 is expressed selectively in brain. Freshly dissected rat tissues were homogenized and analyzed on two identical SDS gels; one was stained with Coomassie blue and the other was immunoblotted consecutively with anti-ykt6 antibody and antimembrin antibody. Detection was with enhanced chemiluminescence. Top two panels show normal (not light) exposures. Bottom panel shows a super-saturating exposure of the anti-ykt6–decorated blot. Similar results for ykt6 on this blot were obtained using the anti-DGH antibody (unpublished data) or using a different blot from a different individual rat (unpublished data).