Figure 13.
Rat ykt6 assembles with other SNAREs similarly to the R-SNARE sec22b. Purified bacterially expressed soluble ytkt6 was mixed with sec22b, syntaxin 5, membrin, and rbet1 in the indicated combinations of proteins. After an overnight incubation at 4°C, the proteins were gel-filtered on Superdex 200, and individual column fractions were immunoblotted with anti-ykt6. Note that rat ykt6 does not bind significantly to any subset of the ER/Golgi Q-SNAREs; it does, however, incorporate into a quaternary complex containing all of them (fractions 16–18, top panel). Elution positions of size calibration proteins are indicated with arrows above the fraction numbers.