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Titers of MB variants of H5N1 virus in the lungs (A) and brains (B) of infected mice. Mice were intranasally inoculated with 0.1 ml of PBS-diluted allantoic fluid containing 101.5 to 102.5 EID50 of infective virus. Virus titers were determined in approximately 10% tissue homogenates prepared in PBS with antibiotic-antimycotic solution. The lungs and brains were removed from mice that were sacrificed at the terminal stage of illness on day 8 after infection.
