Fig. 2. Dynamics of Gem-induced cellular elongation. COS cells transiently expressing GFP–Gem were followed by time-lapse video microscopy for several hours. (A–C) Frames selected at the indicated time points from a representative movie. Arrows in (B) and (C) point at sites of cortical contraction. The arrowhead in (B) indicates the new position of the nucleus. (D) Fluorescence image taken at the same time point as in (A). Cells transfected by GFP–GEM can be identified. (E and F) Schematic representations of the morphological changes that the cells in the centre and at the top right, respectively, underwent. The drawings in red represent the initial cell shapes as seen in (A); the drawings in black represent the final cell shapes as seen in (C).