TABLE 1.
Comparison of Genotype MTBDR test results with DNA sequencing data, INNO-LiPA Rif.TB profiles, and the MICs of RIF in Turkish RIF-resistant isolates (n = 41) of M. tuberculosisa
| Nucleotide/amino acid change(s) | MTBDR profile/result | LiPA profile/result | MIC (μg/ml) (no. of isolates) | No. (%) of isolates |
|---|---|---|---|---|
| 531-TCG→TTG/Ser→Leu | Mut3/resistant | R5/resistant | >32 (17), 32 (2) | 19 (46.3) |
| 531-TCG→TGG/Ser→Trp | ΔWT5/resistant | ΔS5/resistant | >32 (4) | 4 (9.8) |
| 533-CTG→CCG/Leu→Pro | ΔWT5/resistant | ΔS5/resistant | 4 (1) | 1 (2.4) |
| 526-CAC→TAC/His→Tyr | Mut2a/resistant | R4a/resistant | >32 (2) | 2 (4.9) |
| 526-CAC→CGC/His→Arg | ΔWT4/resistant | ΔS4/resistant | >32 (4) | 4 (9.8) |
| 526-CAC→TGC/His→Cys | ΔWT4/resistant | ΔS4/resistant | 8 (1) | 1 (2.4) |
| 522-TCG→TGG/Ser→Trp | ΔWT3/resistant | ΔS3/resistant | >32 (2) | 2 (4.9) |
| 516-GAC→GTC/Asp→Val | Mut1/resistant | R2/resistant | 8 (1) | 1 (2.4) |
| 516-GAC→TAC/Asp→Tyr | ΔWT2/resistant | ΔS2/resistant | 8 (2) | 2 (4.9) |
| 513-CAA→CCA/Gln→Pro | ΔWT1/resistant | ΔS1/resistant | >32 (1) | 1 (2.4) |
| 513-CAA→GAA/Gln→Glu | ΔWT1/resistant | ΔS1/resistant | >32 (1) | 1 (2.4) |
| 515-ATG→ATC/Met→Ile | ΔWT2, ΔWT5/resistant | ΔS2, ΔS5/resistant | 8 (1) | 1 (2.4) |
| 533 CTG→CCG/Leu→Pro | ||||
| CGG insertion between 514 and 515/Arg | S/sensitive | ΔS1, ΔS2/resistant | 32 (1) | 1 (2.4) |
| 490-CAG→CAT/Gln→His | S/sensitive | S/sensitive | >32 (1) | 1 (2.4) |
a
Mut1, Mut2a, Mut3, R2, R4a, and R5 indicate the specific point mutation patterns in the Genotype MTBDR and INNO-LiPA Rif.TB assays, respectively. Δ indicates the negative signal at any of the wild-type probes without a positive signal at the corresponding mutant probe.