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Gcn4 degradation is inhibited in cdc53 and skp1 mutants. Pulse–chase analysis of Gcn4–-LacZ expressed from the CUP1 promoter of plasmid KB449 in strains W303 and MTY740 (left) or YPH1015, YPH1161, and YPH1172 (right). The cultures were shifted from 30 to 37°C, 30 min before labeling.
