Fig. 4.
RNAi-mediated MetAP-2 knockdown. (a) Western blot analysis of MetAP-2 in HEK293 and HUVE cells. Twenty micrograms of cell lysate was fractionated on SDS/PAGE and immunoblotted with anti-MetAP-2 or antiactin antibodies. (b) The effect of MetAP-2 knockdown by RNAi on cell proliferation assessed by [3H]thymidine incorporation. (c) Fg sensitivity of HEK293 cells with RNAi depletion of MetAP-2. (d) Fg sensitivity of HUVE cells with RNAi depletion of MetAP-2. Cell proliferation and Fg sensitivity were determined as described in Materials and Methods. Ctl, control (empty vector). Results are presented as the mean and standard deviation of six separate samples.