Figure 4.
Gradual transformation of MLBs into AVd by leupeptin treatment. GlcNAc-TV-transfected M1 cells were plated for 2 d in regular medium and then incubated for 15 (A and B), 48 (C), 72 (D), or 96 (E) h in the presence of 2 μg/ml leupeptin and processed for electron microscopy. At early times after addition of leupeptin, dense material can be observed at the periphery of MLBs (A and B), and at later times dense vacuoles proliferate such that after 96 h autophagic vacuoles predominate, and MLBs are no longer present. Bars, 0.5 μm.