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'In situ' calibration procedure. A, B: Fluorescence ratio time series of agar beads decorated with Pt-GFP (A) or ratiometric pHluorin (B) during dialysis against buffers of different pH as indicated by the top bar. C, D: Resulting calibration curves. Error bars indicate standard deviation. Data in C are averages of 9 from 7 individual experiments and data in D are averages of 14 from 4 individual experiments.
