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Allele-specific binding of transcription factors. EMSA analysis of allele-specific effect of −118C→A (A), −369C→G (B), and −534G→A (C) on the interaction of nuclear protein complexes extracted from SH-SY5Y cells. DIG-labeled double-stranded probes (200 fmol) were incubated with 10 μg nuclear extract from SH-SY5Y cells. In competition experiments, 50-fold excess of unlabeled probe was added before the addition of the labeled probes.
