TABLE 4.
Duration of antigen-specific recognition by p53 TCR-electroporated PBLs
| Days post-EP (IFN-γ, pg/ml) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Peptide/T2 | EP/PBL | 1 | 2 | 3 | 4 | 5 | 7 | 8 | |
| p53 (264–272) | GFP | 440 (±200) | 510 (±261) | 531 (±115) | 1035 (±1090) | 0 | 85 (±15) | 60 (±14) | |
| p53 | 66,600 (±4640) | 45,141 (±4350) | 33,665 (±2901) | 19,345 (±1985) | 2351 (±275) | 530 (±190) | 152 (±17) | ||
| NY-ESO-1 | GFP | 358 (±86) | 498 (±24) | 551 (±51) | 492 (±140) | 27 (±0) | 55 (±5) | 64 (±28) | |
| p53 | 632 (±124) | 1347 (±129) | 499 (±107) | 421 (±250) | 0 | 45 (±30) | 107 (±10) | ||
| No peptide | GFP | 400 (±294) | 666 (±435) | 1010 (±890) | 505 (±230) | 35 (±0) | 75 (±15) | 57 (±19) | |
| p53 | 440 (±94) | 1218 (±159) | 480 (±56) | 335 (±210) | 60 (±0) | 60 (±10) | 107 (±5) | ||
PBLs were stimulated with OKT3 for 3 days and electroporated with p53-specific TCR or GFP RNA. Electroporated PBLs were cocultured with peptide-pulsed T2 cell line at the days indicated and IFN-γ secretion was detected by ELISA. Values are means of triplicate samples (SD). Data are representative of two experiments.