Fig. 4.
Recurrent deletions involving chromosome 13. FISH analyses of NXc/−p53−/− tumor metaphases were performed by using chromosome 13 BACs. The ideogram at top of figure indicates the position of chromosome 13 BAC probes. All tumors examined had one untranslocated, apparent normal chromosome 13 and one t(13; 6), a complex t(13; 6; 8), or a t(13; 12) marker chromosome. DAPI staining (dark blue) and chromosomes 13 (bright blue), 6 (orange), and 12 (pink) paints are shown. Yellow lightning symbol denotes the chromosome 13 breakpoints. Chromosome 13 translocations consistently result in a single copy loss of the patched genomic locus. Loss of at least a single copy of patched was verified in all tumors analyzed by CGH (Table 1 and Fig. 8, which is published as supporting information on the PNAS web site). Mbp is abbreviated as Mb in figure.