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. 2001 Mar;157(3):1277–1284. doi: 10.1093/genetics/157.3.1277

An amplified fragment length polymorphism map of the silkworm.

Y D Tan 1, C Wan 1, Y Zhu 1, C Lu 1, Z Xiang 1, H W Deng 1
PMCID: PMC1461547  PMID: 11238411

Abstract

The silkworm (Bombyx mori L.) is a lepidopteran insect with a long history of significant agricultural value. We have constructed the first amplified fragment length polymorphism (AFLP) genetic linkage map of the silkworm B. mori at a LOD score of 2.5. The mapping AFLP markers were genotyped in 47 progeny from a backcross population of the cross no. 782 x od100. A total of 1248 (60.7%) polymorphic AFLP markers were detected with 35 PstI/TaqI primer combinations. Each of the primer combinations generated an average of 35.7 polymorphic AFLP markers. A total of 545 (44%) polymorphic markers are consistent with the expected segregation ratio of 1:1 at the significance level of P = 0.05. Of the 545 polymorphic markers, 356 were assigned to 30 linkage groups. The number of markers on linkage groups ranged from 4 to 36. There were 21 major linkage groups with 7-36 markers and 9 relatively small linkage groups with 4-6 markers. The 30 linkage groups varied in length from 37.4 to 691.0 cM. The total length of this AFLP linkage map was 6512 cM. Genetic distances between two neighboring markers on the same linkage group ranged from 0.2 to 47 cM with an average of 18.2 cM. The sex-linked gene od was located between the markers P1T3B40 and P3T3B27 at the end of group 3, indicating that AFLP linkage group 3 was the Z (sex) chromosome. This work provides an essential basic map for constructing a denser linkage map and for mapping genes underlying agronomically important traits in the silkworm B. mori L.

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Selected References

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  1. Becker J., Vos P., Kuiper M., Salamini F., Heun M. Combined mapping of AFLP and RFLP markers in barley. Mol Gen Genet. 1995 Nov 1;249(1):65–73. doi: 10.1007/BF00290237. [DOI] [PubMed] [Google Scholar]
  2. Castiglioni P., Pozzi C., Heun M., Terzi V., Müller K. J., Rohde W., Salamini F. An AFLP-based procedure for the efficient mapping of mutations and DNA probes in barley. Genetics. 1998 Aug;149(4):2039–2056. doi: 10.1093/genetics/149.4.2039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Causse M. A., Fulton T. M., Cho Y. G., Ahn S. N., Chunwongse J., Wu K., Xiao J., Yu Z., Ronald P. C., Harrington S. E. Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics. 1994 Dec;138(4):1251–1274. doi: 10.1093/genetics/138.4.1251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chalhoub B. A., Thibault S., Laucou V., Rameau C., Höfte H., Cousin R. Silver staining and recovery of AFLP amplification products on large denaturing polyacrylamide gels. Biotechniques. 1997 Feb;22(2):216-8, 220. doi: 10.2144/97222bm03. [DOI] [PubMed] [Google Scholar]
  5. Dib C., Fauré S., Fizames C., Samson D., Drouot N., Vignal A., Millasseau P., Marc S., Hazan J., Seboun E. A comprehensive genetic map of the human genome based on 5,264 microsatellites. Nature. 1996 Mar 14;380(6570):152–154. doi: 10.1038/380152a0. [DOI] [PubMed] [Google Scholar]
  6. Dietrich W. F., Miller J., Steen R., Merchant M. A., Damron-Boles D., Husain Z., Dredge R., Daly M. J., Ingalls K. A., O'Connor T. J. A comprehensive genetic map of the mouse genome. Nature. 1996 Mar 14;380(6570):149–152. doi: 10.1038/380149a0. [DOI] [PubMed] [Google Scholar]
  7. Faris J. D., Laddomada B., Gill B. S. Molecular mapping of segregation distortion loci in Aegilops tauschii. Genetics. 1998 May;149(1):319–327. doi: 10.1093/genetics/149.1.319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grattapaglia D., Sederoff R. Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers. Genetics. 1994 Aug;137(4):1121–1137. doi: 10.1093/genetics/137.4.1121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Harushima Y., Yano M., Shomura A., Sato M., Shimano T., Kuboki Y., Yamamoto T., Lin S. Y., Antonio B. A., Parco A. A high-density rice genetic linkage map with 2275 markers using a single F2 population. Genetics. 1998 Jan;148(1):479–494. doi: 10.1093/genetics/148.1.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Heckel D. G., Gahan L. J., Liu Y. B., Tabashnik B. E. Genetic mapping of resistance to Bacillus thuringiensis toxins in diamondback moth using biphasic linkage analysis. Proc Natl Acad Sci U S A. 1999 Jul 20;96(15):8373–8377. doi: 10.1073/pnas.96.15.8373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Herbergs J., Siwek M., Crooijmans R. P., Van der Poel J. J., Groenen M. A. Multicolour fluorescent detection and mapping of AFLP markers in chicken (Gallus domesticus). Anim Genet. 1999 Aug;30(4):274–285. doi: 10.1046/j.1365-2052.1999.00494.x. [DOI] [PubMed] [Google Scholar]
  12. Herrera R. J., Wang J. Evidence for a relationship between the Bombyx mori middle repetitive Bm1 sequence family and U1 snRNA. Genetica. 1991;84(1):31–37. doi: 10.1007/BF00123982. [DOI] [PubMed] [Google Scholar]
  13. Huys G., Coopman R., Janssen P., Kersters K. High-resolution genotypic analysis of the genus Aeromonas by AFLP fingerprinting. Int J Syst Bacteriol. 1996 Apr;46(2):572–580. doi: 10.1099/00207713-46-2-572. [DOI] [PubMed] [Google Scholar]
  14. Knapik E. W., Goodman A., Atkinson O. S., Roberts C. T., Shiozawa M., Sim C. U., Weksler-Zangen S., Trolliet M. R., Futrell C., Innes B. A. A reference cross DNA panel for zebrafish (Danio rerio) anchored with simple sequence length polymorphisms. Development. 1996 Dec;123:451–460. doi: 10.1242/dev.123.1.451. [DOI] [PubMed] [Google Scholar]
  15. Lander E. S., Botstein D. Mapping mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics. 1989 Jan;121(1):185–199. doi: 10.1093/genetics/121.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lander E. S., Green P., Abrahamson J., Barlow A., Daly M. J., Lincoln S. E., Newberg L. A., Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987 Oct;1(2):174–181. doi: 10.1016/0888-7543(87)90010-3. [DOI] [PubMed] [Google Scholar]
  17. Latorra D., Schanfield M. S. Analysis of human specificity in AFLP systems APOB, PAH, and D1S80. Forensic Sci Int. 1996 Nov 11;83(1):15–25. doi: 10.1016/0379-0738(96)02006-3. [DOI] [PubMed] [Google Scholar]
  18. Liu Y. G., Mori N., Tsunewaki K. Restriction fragment length polymorphism (RFLP) analysis in wheat. I. Genomic DNA library construction and RFLP analysis in common wheat. Jpn J Genet. 1990 Oct;65(5):367–380. doi: 10.1266/jjg.65.367. [DOI] [PubMed] [Google Scholar]
  19. Lyons L. A., Laughlin T. F., Copeland N. G., Jenkins N. A., Womack J. E., O'Brien S. J. Comparative anchor tagged sequences (CATS) for integrative mapping of mammalian genomes. Nat Genet. 1997 Jan;15(1):47–56. doi: 10.1038/ng0197-47. [DOI] [PubMed] [Google Scholar]
  20. Lyons L. A., Laughlin T. F., Copeland N. G., Jenkins N. A., Womack J. E., O'Brien S. J. Comparative anchor tagged sequences (CATS) for integrative mapping of mammalian genomes. Nat Genet. 1997 Jan;15(1):47–56. doi: 10.1038/ng0197-47. [DOI] [PubMed] [Google Scholar]
  21. Lyttle T. W. Segregation distorters. Annu Rev Genet. 1991;25:511–557. doi: 10.1146/annurev.ge.25.120191.002455. [DOI] [PubMed] [Google Scholar]
  22. Mackill D. J., Zhang Z., Redoña E. D., Colowit P. M. Level of polymorphism and genetic mapping of AFLP markers in rice. Genome. 1996 Oct;39(5):969–977. doi: 10.1139/g96-121. [DOI] [PubMed] [Google Scholar]
  23. Morizot D. C., Wright D. A., Siciliano M. J. Three linked enzyme loci in fishes: implications in the evolution of vertebrate chromosomes. Genetics. 1977 Jul;86(3):645–656. doi: 10.1093/genetics/86.3.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rouppe van der Voort J. N., van Zandvoort P., van Eck H. J., Folkertsma R. T., Hutten R. C., Draaistra J., Gommers F. J., Jacobsen E., Helder J., Bakker J. Use of allele specificity of comigrating AFLP markers to align genetic maps from different potato genotypes. Mol Gen Genet. 1997 Jul;255(4):438–447. doi: 10.1007/s004380050516. [DOI] [PubMed] [Google Scholar]
  25. Röder M. S., Korzun V., Wendehake K., Plaschke J., Tixier M. H., Leroy P., Ganal M. W. A microsatellite map of wheat. Genetics. 1998 Aug;149(4):2007–2023. doi: 10.1093/genetics/149.4.2007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Simons G., van der Lee T., Diergaarde P., van Daelen R., Groenendijk J., Frijters A., Büschges R., Hollricher K., Töpsch S., Schulze-Lefert P. AFLP-based fine mapping of the Mlo gene to a 30-kb DNA segment of the barley genome. Genomics. 1997 Aug 15;44(1):61–70. doi: 10.1006/geno.1997.4844. [DOI] [PubMed] [Google Scholar]
  27. Ueda H., Mizuno S., Shimura K. Transposable genetic element found in the 5'-flanking region of the fibroin H-chain gene in a genomic clone from the silkworm Bombyx mori. J Mol Biol. 1986 Aug 5;190(3):319–327. doi: 10.1016/0022-2836(86)90004-5. [DOI] [PubMed] [Google Scholar]
  28. Van der Lee T, De Witte I, Drenth A, Alfonso C, Govers F. AFLP Linkage Map of the Oomycete Phytophthora infestans. Fungal Genet Biol. 1997 Jun;21(3):278–291. doi: 10.1006/fgbi.1997.0981. [DOI] [PubMed] [Google Scholar]
  29. Vos P., Hogers R., Bleeker M., Reijans M., van de Lee T., Hornes M., Frijters A., Pot J., Peleman J., Kuiper M. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 1995 Nov 11;23(21):4407–4414. doi: 10.1093/nar/23.21.4407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wada H., Naruse K., Shimada A., Shima A. Genetic linkage map of a fish, the Japanese medaka Oryzias latipes. Mol Mar Biol Biotechnol. 1995 Sep;4(3):269–274. [PubMed] [Google Scholar]
  31. Wang W., Swevers L., Iatrou K. Mariner (Mos1) transposase and genomic integration of foreign gene sequences in Bombyx mori cells. Insect Mol Biol. 2000 Apr;9(2):145–155. doi: 10.1046/j.1365-2583.2000.00172.x. [DOI] [PubMed] [Google Scholar]
  32. Watanabe M., Tomita K., Burioka N., Yajima H., Nakamura H., Ohgi S., Shimizu E. Mucoepidermoid carcinoma of the trachea with airway hyperresponsiveness. Anticancer Res. 2000 May-Jun;20(3B):1995–1997. [PubMed] [Google Scholar]
  33. Waugh R., Bonar N., Baird E., Thomas B., Graner A., Hayes P., Powell W. Homology of AFLP products in three mapping populations of barley. Mol Gen Genet. 1997 Jul;255(3):311–321. doi: 10.1007/s004380050502. [DOI] [PubMed] [Google Scholar]
  34. Xiong Y., Burke W. D., Eickbush T. H. Pao, a highly divergent retrotransposable element from Bombyx mori containing long terminal repeats with tandem copies of the putative R region. Nucleic Acids Res. 1993 May 11;21(9):2117–2123. doi: 10.1093/nar/21.9.2117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Xiong Y., Eickbush T. H. Dong, a non-long terminal repeat (non-LTR) retrotransposable element from Bombyx mori. Nucleic Acids Res. 1993 Mar 11;21(5):1318–1318. doi: 10.1093/nar/21.5.1318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Yasukochi Y. A dense genetic map of the silkworm, Bombyx mori, covering all chromosomes based on 1018 molecular markers. Genetics. 1998 Dec;150(4):1513–1525. doi: 10.1093/genetics/150.4.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Young W. P., Wheeler P. A., Coryell V. H., Keim P., Thorgaard G. H. A detailed linkage map of rainbow trout produced using doubled haploids. Genetics. 1998 Feb;148(2):839–850. doi: 10.1093/genetics/148.2.839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Zhu J. H., Stephenson P., Laurie D. A., Li W., Tang D., Gale M. D. Towards rice genome scanning by map-based AFLP fingerprinting. Mol Gen Genet. 1999 Feb;261(1):184–195. doi: 10.1007/s004380050956. [DOI] [PubMed] [Google Scholar]
  39. al-Janabi S. M., Honeycutt R. J., McClelland M., Sobral B. W. A genetic linkage map of Saccharum spontaneum L. 'SES 208'. Genetics. 1993 Aug;134(4):1249–1260. doi: 10.1093/genetics/134.4.1249. [DOI] [PMC free article] [PubMed] [Google Scholar]

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