Figure 3.
Thymus-derived NKT cells detected several days after transfer. NKT cell–enriched thymocytes were CFSE-labeled and transferred into WT recipients by way of i.v. injection (A, left). 6 d after transfer, organs were harvested and screened for the presence of CFSE+CD1d/α-GalCer+ cells (A, right). These cells were gated on, and analyzed for, CD4 expression (B, middle and right). Some mice received CFSE-labeled cells that were labeled with anti-CD4 before transfer (B, right). Data are representative of six (A) or two (B) experiments with one or two mice/group.