Table 4.
Validation of haplotype-estimating algorithm. Known haplotype frequencies present in artificial mixtures of MSP-119 clones were compared to maximum likelihood estimates of haplotype frequencies generated using the algorithm.
| Mixture | Actual Haplotype (%) | Maximum Likelihood Estimate Haplotype (%) | ||||||||
| 1 | 2 | 3 | 4 | 5 | 1 | 2 | 3 | 4 | 5 | |
| 1 | QKSNGL (70) | EKSNGL (30) | QKSNGL (73) | EKSNGL (27) | ||||||
| 2 | QKSNGL (30) | EKSNGL (70) | QKSNGL (37) | EKSNGL (63) | ||||||
| 3 | QKSNGL (70) | ETSNGL (30) | QKSNGL (72) | ETSNGL (28) | ||||||
| 4 | QKSNGL (30) | ETSNGL (70) | QKSNGL (33) | ETSNGL (67) | ||||||
| 5 | EKSSRL (70) | ETSNGL (30) | EKSSRL (70) | ETSNGL (30) | ||||||
| 6 | EKSSRL (30) | ETSNGL (70) | EKSSRL (29) | ETSNGL (63) | EKSNGF (07) | |||||
| 7 | QKSNGF (70) | EKSSRL (30) | QKSNGF (75) | EKSSRL (25) | ||||||
| 8 | QKSNGF (30) | EKSSRL (70) | QKSNGF (38) | EKSSRL (62) | ||||||
| 9 | ETSSRL (35) | QKNNGL (15) | QKSNGL (50) | ETSSRL (31) | QKNNGL (14) | QKSNGL (55) | ||||
| 10 | ETSSRL (15) | QKNNGL (35) | QKSNGL (50) | ETSSRL (14) | QKNNGL (38) | QKSNGL (49) | ||||
| 11 | QKSNGL (47) | EKSNGL (20) | EKSSRL (33) | QKSNGL (50) | EKSNGL (21) | EKSSRL (29) | ||||
| 12 | QKSNGL (20) | EKSNGL (47) | EKSSRL (33) | QKSNGL (21) | EKSNGL (49) | EKSSRL (31) | ||||
| 13 | ETSSRL (23) | QKNNGL (10) | QKSNGL (33) | EKSNGL (33) | ETSSRL (21) | QKNNGL (15) | QKSNGL (31) | EKSNGL (33) | ||
| 14 | ETSSRL (10) | QKNNGL (23) | QKSNGL (33) | EKSNGL (33) | ETSSRL (10) | EKNNGL (30) | QKSNGL (60) | |||
| 15 | QKSNGL (31) | EKSNGL (13) | EKSSRL (22) | ETSSRL (33) | EKSNGL (49) | EKSSRL (18) | QTSSRL (33) | |||
| 16 | QKSNGL (13) | EKSNGL (31) | EKSSRL (22) | ETSSRL (33) | QKSNGL (14) | ETSNGL (33) | EKSSRL (53) | |||
| 17 | ETSSRL (15) | QKNNGL (07) | QKSNGL (22) | EKSNGL (22) | QKSNGF (33) | QTSSRL (14) | QKNNGL (07) | QKSNGL (47) | EKSNGF (32) | |
| 18 | QKSNGL (21) | EKSNGL (09) | EKSSRL (15) | ETSSRL (22) | QKSNGF (33) | ETSNGL (17) | EKSNGL (22) | QKSSRL (31) | QKSNGF (30) | |
* Haplotypes indicated in bold type are not present and represent errors in haplotype estimation.