Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Pyrosequencing primers used to genotype polymorphisms in MSP-119.
| SNP | Primer | ||
| Location | Nucleotides | Amino Acids | |
| 1644 | G/C | E/Q | 5'-GCGTAAAAAAACAATGTC-3' |
| 1691 | A/C | K/T | 5'-GTGATGCAGATGCCA-3' |
| 1699a | G/A | S/N | 5'-CCGAAGAAGATTCAGGTA-3' |
| 1700a | G/A | S/N | |
| 1701a | G/A | G/R | |
| 1716b | C/T | L/F | 5'-TCACATGTGAATGTACTAAA-3' |
aSNPs at these three positions are genotyped in the same Pyrosequencing reaction.
b Primer sits down prior to position 1711 where there is a rare polymorphism not considered in this analysis.
