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. 1979 Sep;38(1):23–31.

T and B lymphocytes in pituitary dwarf Snell-Bagg mice.

F Dumont, F Robert, P Bischoff
PMCID: PMC1457905  PMID: 315916

Abstract

The lymphocyte composition of the thymus and spleen from weaned (4 month old) hypopituitary dwarf Snell-Bagg mice were compared to those of their phenotypically normal littermates and of hormone (somatotropic hormone plus thyroxine)-treated individuals. Detection of cells bearing receptors for peanut agglutinin, physical analysis and measurement of in vitro reactivities to phytohaemagglutinin and concanavae intra-thymic lymphocyte population of dwarf mice. Examination of spleen-cell suspensions demonstrated a slightly higher frequency of T lymphocytes (Thy 1-2+ alpha-Naphthyl esterase+, high electrophoretic mobility) and lower frequency of B lymphocytes (surface immunoglobulin+, low electrophoretic mobility) in dwarf mice than in control mice. The degree of splenocyte responsiveness to T- and B-cell mitogens, however was similar in the two mouse types. High mobility (T) splenic cells were found to exhibit a smaller modal volume in dwarf mice (110 micron3) than in control mice (122 micron3) but this difference was not corrected by hormone administration. More pronounced were the quantitative differences between the spleens of hormone-deficient and normal mice. Thus, when expressed as a function of body weight, the numbers of splenic T and B lymphocytes in untreated dwarf mice were about half the corresponding values in hormone-reconstituted or normal littermates. These data suggested that in adult life, developmental hormones exert little direct effect on the thymus lymphocytes but influence the size of the pool of both peripheral T and B lymphocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson J., Möller G., Sjöberg O. Selective induction of DNA synthesis in T and B lymphocytes. Cell Immunol. 1972 Aug;4(4):381–393. doi: 10.1016/0008-8749(72)90040-8. [DOI] [PubMed] [Google Scholar]
  2. Andersson L. C., Nordling S., Häyry P. Fractionation of mouse T and B lymphocytes by preparative cell electrophoresis. Efficiency of the method. Cell Immunol. 1973 Aug;8(2):235–248. doi: 10.1016/0008-8749(73)90113-5. [DOI] [PubMed] [Google Scholar]
  3. Baroni C. D., Scelsi R., Mingazzini P. L., Cavallero A., Uccini S. Delayed hypersensitivity in the hereditary pituitary dwarf snell-bagg mouse. Nat New Biol. 1972 Jun 14;237(76):219–220. doi: 10.1038/newbio237219a0. [DOI] [PubMed] [Google Scholar]
  4. Baroni C. Thymus, peripheral lymphoid tissues and immunological responsiveness of the pituitary dwarf mouse. Experientia. 1967 Apr 15;23(4):282–283. doi: 10.1007/BF02135688. [DOI] [PubMed] [Google Scholar]
  5. Dumont F., Bischoff P. Developmental changes in the electrophoretic mobility of mouse splenic lymphoid cells. Ann Immunol (Paris) 1977 Jul-Oct;128C(4-5):771–784. [PubMed] [Google Scholar]
  6. Dumont F., Nardelli J. Peanut agglutinin (PNA)-binding properties of murine thymocyte subpopulation. Immunology. 1979 May;37(1):217–224. [PMC free article] [PubMed] [Google Scholar]
  7. Dumont F. Physical subpopulations of mouse thymocytes: changes during regeneration subsequent to cortisone treatment. Immunology. 1978 May;34(5):841–852. [PMC free article] [PubMed] [Google Scholar]
  8. Dumont F., Robert F. Changes in the electrophoretic mobility of mouse lymph node lymphocytes during ontogeny. Immunology. 1977 Jun;32(6):989–996. [PMC free article] [PubMed] [Google Scholar]
  9. Duquesnoy R. J., Ahrens R. C. Increased responsiveness to phytohemagglutinin and graft-vs-host reactivity of thymus cells from Snell-Bagg dwarf mice. J Immunol. 1973 Feb;110(2):578–580. [PubMed] [Google Scholar]
  10. Duquesnoy R. J., Kalpaktsoglou P. K., Good R. A. Immunological studies of the Snell-Bagg pituitary dwarf mouse. Proc Soc Exp Biol Med. 1970 Jan;133(1):201–206. doi: 10.3181/00379727-133-34440. [DOI] [PubMed] [Google Scholar]
  11. Fabris N., Pierpaoli W., Sorkin E. Hormones and the immunological capacity. 3. The immunodeficiency disease of the hypopituitary Snell-Bagg dwarf mouse. Clin Exp Immunol. 1971 Aug;9(2):209–225. [PMC free article] [PubMed] [Google Scholar]
  12. Fabris N., Pierpaoli W., Sorkin E. Hormones and the immunological capacity. IV. Restorative effects of developmental hormones or of lymphocytes on the immunodeficiency syndrome of the dwarf mouse. Clin Exp Immunol. 1971 Aug;9(2):227–240. [PMC free article] [PubMed] [Google Scholar]
  13. Kemp R. G., Huang Y. C., Duquesnoy R. J. Decreased epinephrine response of adenylate cyclase activity of lymphoid cells from immunodeficient pituitary dwarf mice. J Immunol. 1973 Dec;111(6):1855–1861. [PubMed] [Google Scholar]
  14. London J., Berrih S., Bach J. F. Peanut agglutinin. I. A new tool for studying T lymphocyte subpopulations. J Immunol. 1978 Aug;121(2):438–443. [PubMed] [Google Scholar]
  15. Moon R., Phillips R. A., Miller R. G. Sedimentation and volume analysis of human bone marrow. Ser Haematol. 1972;5(2):163–178. [PubMed] [Google Scholar]
  16. Mueller J., Brun del Re G., Buerki H., Keller H. U., Hess M. W., Cottier H. Nonspecific acid esterase activity: a criterion for differentiation of T and B lymphocytes in mouse lymph nodes. Eur J Immunol. 1975 Apr;5(4):270–274. doi: 10.1002/eji.1830050411. [DOI] [PubMed] [Google Scholar]
  17. Pelletier M., Montplaisir S., Dardenne M., Bach J. F. Thymic hormone activity and spontaneous autoimmunity in dwarf mice and their littermates. Immunology. 1976 Jun;30(6):783–788. [PMC free article] [PubMed] [Google Scholar]
  18. Pierpaoli W., Baroni C., Fabris N., Sorkin E. Hormones and immunological capacity. II. Reconstitution of antibody production in hormonally deficient mice by somatotropic hormone, thyrotropic hormone and thyroxin. Immunology. 1969 Feb;16(2):217–230. [PMC free article] [PubMed] [Google Scholar]
  19. Pierpaoli W., Fabris N., Sorkin E. Developmental hormones and immunological maturation. Ciba Found Study Group. 1970;36:126–153. [PubMed] [Google Scholar]
  20. Raff M. C., Owen J. J. Thymus-derived lymphocytes: their distribution and role in the development of peripheral lymphoid tissues of the mouse. Eur J Immunol. 1971 Jan;1(1):27–30. doi: 10.1002/eji.1830010105. [DOI] [PubMed] [Google Scholar]
  21. Raff M. C., Sternberg M., Taylor R. B. Immunoglobulin determinants on the surface of mouse lymphoid cells. Nature. 1970 Feb 7;225(5232):553–554. doi: 10.1038/225553a0. [DOI] [PubMed] [Google Scholar]
  22. Ranki A., Tötterman T. H., Häyry P. Identification of mouse T and B lymphocytes from cytocentrifuged cell smears. Clin Exp Immunol. 1976 Dec;26(3):632–640. [PMC free article] [PubMed] [Google Scholar]
  23. Shire J. G. Growth hormone and premature ageing. Nature. 1973 Sep 28;245(5422):215–216. doi: 10.1038/245215a0. [DOI] [PubMed] [Google Scholar]
  24. Snell G. D. DWARF, A NEW MENDELIAN RECESSIVE CHARACTER OF THE HOUSE MOUSE. Proc Natl Acad Sci U S A. 1929 Sep 15;15(9):733–734. doi: 10.1073/pnas.15.9.733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sultzer B. M., Nilsson B. S. PPD tuberculin--a B-cell mitogen. Nat New Biol. 1972 Dec 13;240(102):198–200. doi: 10.1038/newbio240198a0. [DOI] [PubMed] [Google Scholar]
  26. Wilkinson P. C., Singh H., Sorkin E. Serum immunoglobulin levels in thymus-deficient pituitary dwarf mice. Immunology. 1970 Mar;18(3):437–441. [PMC free article] [PubMed] [Google Scholar]
  27. Zeiller K., Löser R., Pascher G., Hannig K. Free-flow electrophoresis. II. Analysis of the method with respect to preparative cell separation. Hoppe Seylers Z Physiol Chem. 1975 Aug;356(8):1225–1244. doi: 10.1515/bchm2.1975.356.2.1225. [DOI] [PubMed] [Google Scholar]
  28. Zeiller K., Pascher G., Hannig K. B lymphocyte subpopulations in the mouse spleen. A study of the differentiation pathway using free flow electrophoretically separated subpopulations of direct PFC progenitor cells. Immunology. 1976 Dec;31(6):863–880. [PMC free article] [PubMed] [Google Scholar]

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