Figure 3.
Flies containing the dSmg1rst2 mutant do not have impaired nonsense-mediated decay of Adhfn6 null allele transcripts. Real-time quantification of Adh and Gapdh transcripts using dual-labeled fluorogenic gene probes (Adh probe 6-FAM-CTGGCGAAACTGGCCCCCATTA-TAMRA, Gapdh probe TET-CCGCTTGGTTCTCCGCGCC-BHQ) in w1118, Adhfn6, and dSmg1rst2; Adhfn6 flies. Adh transcript levels in Adhfn6 and dSmg1rst2; Adhfn6 flies are similar and much lower than those in w1118. The housekeeper control (Gapdh) transcript levels are the same in the three strains. Each combination of primer set and DNA was done in triplicate.