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High-copy GIC1 and BNI1 suppress the temperature-sensitive phenotype of a pkc1 (stt1) mutant. Tenfold dilutions of the pkc1 (stt1) strain (SVL170) harboring plasmids pSV181 (PKC1), pRS426 (vector), pSV294 (BCK1-20), or pSV304 (GIC1) were grown for 3–4 days on SC plates at 25° and 37°.
