Abstract
Bacteriophage has been coupled to the monovalent antibody fragment Fab' made by enzymatic digestion of antiphage antibody. Concomitant neutralization of the phage (T4) was slight under the conditions used. The phage could then be neutralized by anti-Fab sera. This method has been used to assay for anti-Fab antibodies and found to have a threshold sensitivity of about 6 ng antibody/ml. It is hoped to couple other protein antigens to phage by prior linkage to anti-phage Fab′.
A method for the rapid titration of large numbers of anti-phage and anti-Fab sera using a phage applicator is described.
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- Carter B. G., Yo S. L., Sehon A. H. The use of bacteriophage neutralization technique for the detection of anti-hapten antibody. Can J Biochem. 1968 Mar;46(3):261–265. doi: 10.1139/o68-037. [DOI] [PubMed] [Google Scholar]
- Haimovich J., Sela M., Dewdney J. M., Batchelor F. R. Anti-penicilloyl antibodies: detection with penicilloylated bacteriophage and isolation with a specific immunoadsorbent. Nature. 1967 Jun 24;214(5095):1369–1370. doi: 10.1038/2141369a0. [DOI] [PubMed] [Google Scholar]
- Haimovich J., Sela M. Inactivation of poly-DL-alanyl bacteriophage T4 with antisera specific toward poly-DL-alanine. J Immunol. 1966 Sep;97(3):338–343. [PubMed] [Google Scholar]
- Mäkelä O. Assay of anti-hapten antibody with the aid of hapten-coupled bacteriophage. Immunology. 1966 Jan;10(1):81–86. [PMC free article] [PubMed] [Google Scholar]
- Stemke G. W., Lennox E. S. Bacteriophage neutralizing activity of fragments derived from rabbit immunoglobulin by papain digestion. J Immunol. 1967 Jan;98(1):94–101. [PubMed] [Google Scholar]
- TARR H. A. Mechanical aids for the phage-typing of Staphylococcus aureus. Mon Bull Minist Health Public Health Lab Serv. 1958 Mar;17:64–72. [PubMed] [Google Scholar]