FIGURE 2.
EMSAs performed with EBER 1 deletion constructs identify stem–loops I, III, and IV as binding sites for L22 on EBER 1. Predicted secondary structures of the EBER 1 deletion RNAs tested (top) and EMSAs performed with 5 nM RNA (body-labeled) and increasing concentrations of L22 (bottom). (A) Full-length EBER 1, (B) EBER 1 Δstlp I (Δnts 14–33), (C) EBER 1 Δstlp II (Δnts 45–58), (D) EBER 1 Δstlp III (Δnts 60–83), (E) EBER 1 Δstlp IV (Δnts 87–123). The deletion mutants are numbered according to the full-length RNA. An asterisk (*) indicates an additional, nonspecific shift that only appears at L22 concentrations ≥3 μM.