FIG. 6.
Protein production patterns of selected proteins over the course of Streptococcus pneumoniae serotype 3 biofilm development. Representative production patterns of glucose-6-phosphate isomerase (A), glycl-tRNA synthetase (B), and pneumolysin (C). The production pattern analysis was carried out from 2D images that were scanned using a calibrated image scanner (GE Healthcare). Image analysis was done using the Image Master 2D Platinum software (GE Healthcare). Computational analysis was based on 2D protein spot volume. The planktonic growth stage and three biofilm developmental stages were analyzed thus: planktonic, planktonic cell stage; 3 days, 3-day-old biofilms; 6 days, 6-day-old biofilms, and 9 days, 9-day-old biofilms. The data shown here represent the average spot volumes. Experiments were carried out in triplicate for each growth stage.