TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype/phenotype | Origin/reference |
|---|---|---|
| E. coli strains | ||
| DH5α | F−lacZΔM15 Δ(lacZYA-argF)U169; wild type | Gibco BRL |
| BL21(DE3)pLysS | F−ompT hsdS (r− m−) gal dcm (DE3) pLysS; Cmr | Stratagene |
| AH1646 | F−ompT hsdS (r− m−) gal dcm (DE3) pLysS pGR28; Ampr Cmr | This work |
| AH3268 | F−ompT hsdS (r− m−) gal dcm (DE3) pLysS pGR144; Ampr Cmr | This work |
| B. subtilis strains | ||
| MB24 | trpC2 metC3; wild type | Laboratory stock |
| SL666 | trpC2 spoVE85 | 26 |
| AH1744 | trpC2 metC3 amyE::PxylA-murB(77-753); Neor | This work |
| AH1745 | trpC2 metC3 amyE::PxylA-murB; Cmr | This work |
| AH1746 | trpC2 metC3 murB::neo amyE::PxylA-murB; Neor Cmr | This work |
| AH3356 | trpC2 metC3 murB::neo amyE::PxylA-murB thrC::Pspac-divIB; Neor Cmr Emr | This work |
| AH3497 | trpC2 metC3 ΔmurB amyE::PxylA-murB; Cmr | This work |
| AH3505 | trpC2 metC3 ΔmurB amyE::PxylA-murB spβ::PgerE-lacZ; Cmr Emr | This work |
| Plasmids | ||
| pBEST501 | bla neo | 32 |
| pMS38 | bla cm | 59 |
| pDG364 | bla amyE::cm | 8 |
| pMLK83 | bla amyE::gus neo | 36 |
| pAH235 | bla amyE::PspoIIIG-gusA neo | 28 |
| pAH256 | bla spec | 28 |
| pMALc2 | bla malE | New England Biolabs |
| pCR2.1-TOPO | bla | Invitrogen, La Jolla, CA |
| pGEM T Easy | bla | Promega |
| pGR28 | bla malE-murB | This work |
| pGR29 | bla PxylA | This work |
| pGR34 | bla murB::neo | This work |
| pGR35 | bla amyE::PxylA-gusA neo | This work |
| pGR36 | bla amyE::PxylA-murB(77-753) neo | This work |
| pGR40 | bla amyE::PxylA neo | This work |
| pGR43 | bla murB(301-1597) cm | This work |
| pGR46 | bla murB(77-753) | This work |
| pGR63 | bla amyE::Pspac-divIB neo | 45 |
| pGR144 | bla malE-divIB | This work |
| pGR163 | bla thrC::Pspac-divIB erm, spec | This work |
| pGR194 | bla ΔmurB | This work |