Abstract
Two factors essential for the lysis of EAC′142 cells are described and designated as β and d. These factors are distinct from the C′3-subunits a, b and c described earlier; they can be separated by chemical procedures. A simple assay method for both factors is given. Four highly reactive intermediates, EAC′142,a; EAC′142,a,b; EAC′142,a,b,β and EAC′142,a,b,β,c can be prepared and characterized by their lytic reactivity pattern against different systems of incomplete C′3. Evidence is presented that the subunits of the C′3-system interact with EAC′142 in a sequential order corresponding to the formula a—b—β—c—d.
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Selected References
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- Klein P. G., Wellensiek H. J. Multiple nature of the third component of guinea-pig complement. I. Separation and characterization of three factors a, b, and c, essential for haemolysis. Immunology. 1965 Jun;8(6):590–603. [PMC free article] [PubMed] [Google Scholar]
- LINSCOTT W. D., NISHIOKA K. COMPONENTS OF GUINEA PIG COMPLEMENT. II. SEPARATION OF SERUM FRACTIONS ESSENTIAL FOR IMMUNE HEMOLYSIS. J Exp Med. 1963 Nov 1;118:795–815. doi: 10.1084/jem.118.5.795. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MAYER M. M., LEVINE L., RAPP H. J., MARUCCI A. A. Kinetic studies on immune hemolysis. VII. Decay of EAC'1, 4, 2, fixation of C'3, and other factors influencing the hemolytic action of complement. J Immunol. 1954 Dec;73(6):443–454. [PubMed] [Google Scholar]