Table 2.
Antigenic analysis of H5N1 influenza viruses from different regions in Asia by HA inhibition titer
| Virus | Sublineage* | mAb to Ck/PA/1370/83 |
mAb to Ck/HK/YU22/02 |
mAb to VNM/1203/04 |
Antisera |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CP25 | CP58 | CP176/26 | 3C8 | 7C6 | 8H11 | 10H4D2 | 15A3 | 1203/04 | MS/04 | 437.4/99 | ||
| Ck/PA/1370/83 | Reference | 12,800 | 12,800 | 12,800 | 12,800 | 12,800 | 12,800 | 12,800 | 12,800 | 160 | 80 | 80 |
| Gs/HK/437.10/99 | 3,200 | 12,800 | 12,800 | 800 | 12,800 | 12,800 | 12,800 | 12,800 | 1,280 | < | 1280 | |
| Dk/HK/2986.1/00 | 1,600 | 6,400 | 1,600 | 6,400 | 12,800 | 12,800 | 12,800 | 12,800 | 1,280 | < | 640 | |
| Ck/HK/YU22/02 | 200 | < | 200 | 6,400 | 1,600 | 1,600 | 12,800 | 12,800 | < | 80 | 640 | |
| Dk/HN/5806/03 | HN | < | 1,600 | 12,800 | 12,800 | 800 | 12,800 | 12,800 | < | < | 80 | 320 |
| Ck/HN/999/05 | < | 200 | 12,800 | 6,400 | 1,600 | 6,400 | 12,800 | < | < | 80 | 80 | |
| Dk/YN/6445/03 | YN | < | < | 200 | 3,200 | 400 | 800 | < | 200 | < | 160 | 320 |
| Ck/YN/115/04 | < | 200 | 400 | 6,400 | 800 | 1,600 | 400 | 400 | < | 80 | 320 | |
| Ck/YN/447/05 | < | 1,600 | 1,600 | 1,600 | < | 400 | < | < | < | < | < | |
| Ck/Indonesia/BL/03 | IDN | < | < | 800 | 12,800 | 1,600 | 12,800 | < | 800 | < | 320 | 640 |
| Dk/Indonesia/MS/04 | < | < | 400 | 12,800 | 1,600 | 3,200 | < | 400 | < | 160 | 640 | |
| Ck/Wajo/BBVM/05 | < | < | 400 | < | 400 | 1600 | < | < | < | 160 | 320 | |
| Vietnam/1194/04 | VTM | 400 | 200 | < | 12,800 | 6,400 | < | 12,800 | 12,800 | 2,560 | < | 640 |
| Vietnam/1203/04 | 800 | 400 | < | 6,400 | 6,400 | < | 12,800 | 12,800 | 1,280 | < | 1,280 | |
| Thailand/1(Kan-1)/04 | < | < | < | 12,800 | 12,800 | < | 12,800 | 12,800 | 5,120 | < | 1,280 | |
| Dk/Vietnam/11/04 | 400 | 12,800 | < | 6,400 | 3,200 | 800 | 12,800 | 12,800 | 1,280 | < | 640 | |
| Dk/Vietnam/S654/05 | < | < | < | 800 | 400 | < | 1,600 | 200 | 160 | < | 160 | |
| Dk/Vietnam/568/05 | VNM2 | < | < | < | < | < | < | < | < | < | < | < |
| HK/212/03 | GD | 800 | 200 | 200 | 12,800 | 12,800 | 800 | 12,800 | 12,800 | 160 | 320 | 2,560 |
| Gs/GX/345/05 | Mixed | < | < | < | 6,400 | < | 200 | < | 200 | < | < | < |
| Ck/ST/810/05 | < | < | < | 800 | < | < | < | < | < | < | < | |
| MDk/JX/1653/05 | MB | 400 | 800 | 200 | < | 3,200 | 3,200 | 6,400 | 6,400 | < | < | 640 |
| MDk/JX/2136/05 | < | 800 | 12,800 | 12,800 | 400 | 12,800 | 200 | 200 | < | < | 160 | |
| BH goose/QH/65/05 | < | 800 | 12,800 | 12,800 | 400 | 12,800 | < | < | < | < | < | |
<, lowest dilution tested: 1:100 for monoclonal antibody titres, 1:40 for ferret and chicken anti-sera. 437.4/99, Gs/HK/437.4/99; 1203/04, VNM/1203/04; GD; Guangdong; HN, Hunan; IDN, Indonesia; MB, migratory bird; MS/04, Dk/IDN/MS/04; VNM2, recent Vietnam introduction; VTM, Vietnam/Thailand/Malaysia; YN, Yunnan. Underlined numbers indicate titers to prototype viruses.
*Viruses were selected from each of the sub-sublineages as defined by phylogenetic analysis of the HA gene (Fig. 2A).