Table 1.
List of antibodies used
|
Antigen |
Antibody |
Species |
Clone/name |
Dilution |
Source |
|---|---|---|---|---|---|
| α-Dystroglycan | mAb | Mouse | IIH6 | 1:100 | K.Campbell |
| β-Dystroglycan | mAb | Mouse | 43DAG1/8DS | 1:50 | Novocastra Laboratories (Newcastle upon Tyne, UK) |
| Dystrophin Dp 116 | mAb | Mouse | MANDRA1 | 1:100 | Sigma (St. Louis, MO) |
| DRP2 | pAb | Rabbit | 2164 | 1:100 | Peter Brophy (University of Edinburgh, Edinburgh, UK) |
| Ezrin | pAb | Rabbit | 1:30 | Upstate Biotechnology (Lake Placid, NY) | |
| ERM phosphorylated | pAb | Rabbit | 1:50 | Cell Signaling (Beverly, MA) | |
| Caspr | mAb | Mouse | 1:100 | Elior Peles (Weizmann Institute of Science, Rehovot, Israel) | |
| Caspr | pAb | Rabbit | 1:600 | Elior Peles | |
| Laminin α1 | mAb | Rat | 317 | 1:2 | Lydia Sorokin (Lund University, Lund, Sweden) |
| Laminin α2 | mAb | Rat | 4H8/2 | 1:100 | Alexis (San Diego, CA) |
| Laminin α4 | pAb | Rabbit | 1:100 | Jeffrey Miner (Washington University, St. Louis, MO) | |
| Laminin α5 | pAb | Rabbit | 1:400 | Lydia Sorokin | |
| Laminin β2 | pAb | Rabbit | 1117 | 1:50 | Rupert Timpl (Max Plank Institute, Martinsried, Germany) |
| Laminin γ1 | mAb | Rat | A5 | 1:50 | Chemicon (Temecula, CA) |
| MAG | mAb | Mouse | 1:50 | Boehringer Mannheim (Mannheim, Germany) | |
| Neurofascin 155 + 186 | pAb | Rabbit | 1:1000 | Peter Brophy | |
| Potassium channel Kv1.1 | pAb | Rabbit | 1:100 | Alomone Labs (Jerusalem, Israel) | |
| Radixin | pAb | Rabbit | 92-4 | 1:100 | Heinz Furthmayr (Stanford University, Stanford, CA) |
| Sodium channel Nav 1.6 | pAb | Rabbit | 1:200 | James Trimmer (University of California, Davis, CA) | |
| Sodium channel Nav1.2 | mAb | Mouse | K69/3 | 1:100 | Upstate Biotechnology |
| Sodium channel Pan Nav | mAb | Mouse | K58/35 | 1:100 | Sigma |
| Utrophin |
mAb |
Mouse |
DRP3/20C5 |
1:50 |
Novocastra Laboratories |
mAb, Monoclonal antibody; pAb, polyclonal antibody.