Table II. Oligomerization of NcKin and Y362 point mutants.
| Construct | Sw,20 (s–1) | rStokes (nm) | Derived molecular mass (kDa) | Predicted mass from sequence (kDa) | Oligomerization state |
|---|---|---|---|---|---|
| NcKin433 | 4.3 | 4.7 | 83.4 | 47.9 | Dimer |
| NcKin433-Y362K | 3.3 | 4.0 | 54.5 | 47.9 | Monomer |
| NcKin433-Y362C | 3.6 | 3.8 | 56.5 | 47.9 | Monomer |
| NcKin433-Y362F | 3.5 | 3.7 | 53.5 | 47.9 | Monomer |
| NcKin433-F359C | 4.7 | 4.3 | 83.4 | 47.9 | Dimer |
| NcKin383 | 4.0 | 3.0 | 49.5 | 42.7 | Monomer |
| NcKin383-Y362K | 3.6 | 3.2 | 47.6 | 42.7 | Monomer |
Novel mutants were tested on two or three independent preparations.