. 2003 Feb 3;22(3):450–458. doi: 10.1093/emboj/cdg036

Table I. Gliding velocities and steady state ATPase activities of NcKin wild-type and relevant mutants.

Construct	Gliding velocity (µm/s)	k_cat (s^–1)	K_m,ATP (µM)	Basal activity k₀ (s^–1)
NcKin433 (wild type)	2.2 ± 0.2	74 ± 8	90 ± 13	0.017 ± 0.001
NcKin433-Y362K	1.0 ± 0.2	262 ± 7	90 ± 10	0.027 ± 0.000
NcKin433-Y362C	–^a	176 ± 52	ND	ND
NcKin433-Y362F	1.6 ± 0.3	32 ± 15	ND	ND
NcKin433-F359C	–^a	66 ± 31	ND	ND
NcKin383	0.8 ± 0.2^b	24 ± 4^b	ND	ND
NcKin383-Y362K	–^a	157 ± 0	ND	ND

The values are averages of at least two independent measurements.

^aBiotinylation impossible.