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. 2003 Feb;23(3):933–949. doi: 10.1128/MCB.23.3.933-949.2003

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Copyright © 2003, American Society for Microbiology

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FIG. 2. — R-Ras enhances FAK and p130^Cas phosphorylation, leading to an enhancement of cell migration. (A) Expression of activated R-Ras(38V) enhances FAK and p130^Cas phosphorylation. Cells were stimulated with collagen in suspension for 10 min; FAK and p130^Cas were immunoprecipitated (IP) and then immunoblotted with antiphosphotyrosine antibody, anti-FAK, or anti-p130^Cas as indicated. Data shown are representative of more than four experiments. (B and C) R-Ras-induced migration is dependent on FAK and p130^Cas. Cells were transiently transfected with GFP and dominant-negative FAK (FRNK) or p130^Cas (Cas-SH3), and migration was determined in a haptotactic assay. Data are expressed relative to control cells transfected with vector alone plus or minus standard error of the mean (from three separate experiments). *, P ≤ 0.02; **, P ≤ 0.005. FRNK and Cas-SH3 significantly inhibit R-Ras(38V)-induced cell migration. (D) Focal adhesion formation requires FAK and p130^Cas. Cells transiently transfected with FRNK and Cas-SH3 were immunostained with phosphotyrosine as a marker of focal adhesions and were assessed for focal adhesion formation. The graph represents the percentage of 100 transfected cells able to form focal adhesions.

FIG. 2. — R-Ras enhances FAK and p130^Cas phosphorylation, leading to an enhancement of cell migration. (A) Expression of activated R-Ras(38V) enhances FAK and p130^Cas phosphorylation. Cells were stimulated with collagen in suspension for 10 min; FAK and p130^Cas were immunoprecipitated (IP) and then immunoblotted with antiphosphotyrosine antibody, anti-FAK, or anti-p130^Cas as indicated. Data shown are representative of more than four experiments. (B and C) R-Ras-induced migration is dependent on FAK and p130^Cas. Cells were transiently transfected with GFP and dominant-negative FAK (FRNK) or p130^Cas (Cas-SH3), and migration was determined in a haptotactic assay. Data are expressed relative to control cells transfected with vector alone plus or minus standard error of the mean (from three separate experiments). *, P ≤ 0.02; **, P ≤ 0.005. FRNK and Cas-SH3 significantly inhibit R-Ras(38V)-induced cell migration. (D) Focal adhesion formation requires FAK and p130^Cas. Cells transiently transfected with FRNK and Cas-SH3 were immunostained with phosphotyrosine as a marker of focal adhesions and were assessed for focal adhesion formation. The graph represents the percentage of 100 transfected cells able to form focal adhesions.