FIG. 4.
aa 1394 to 2160 of N320 mediate the interaction with C180. (A) Flag-tagged wt or truncated N-terminal MLL constructs were expressed in 293T cells either alone or in combination with Myc-tagged C180. (B to E) Lane 1 of each experiment contained extracts prepared from untransfected cells. Doubly tagged MLL (Flag-MLL-Myc) was in lane 2 throughout. (E) Anti-Flag immunoprecipitation (IP) followed by anti-Myc Western blot analysis indicated that MLL aa 1 to 2160 but not 1 to 1393 efficiently interact with Myc-p180 (lanes 3 and 4).