FIG. 9.
Down-regulation of the MKK6 3′-UTR transcript levels by activation of the p38 MAPK pathway. (A) HEK293 cells were transiently cotransfected with plasmids carrying lacZ MKK6 3′-UTR, pEGFP, and either pEFmlink-MKK6-DD or pEFmlink alone. The amounts of the lacZ MKK6 3′-UTR (upper panel) and GAPDH RNAs were determined by both Northern blotting and RT-PCR. (B) Quantification of the β-galactosidase activity in whole-cell extracts from the same experiment. Data are normalized for the efficiency of transfection, as determined by NIH image analysis of green fluorescent protein (GFP) immunoblots (upper panel). The quantification of two independent experiments is presented.