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MKK6 protein stability is not affected in p38α-deficient cells. Wild-type (wt) and p38α−/− cells were incubated with cycloheximide (CHX, 25 μg/ml) for 4 and 12 h. Cell extracts were analyzed by immunoblotting for expression of p38α, MKK6, tubulin, and cyclin D1.
